Peptide Bond Formation Between the Hetrosubunits of ω-Transaminase, Alanine Dehydrogenase, and Formate Dehydrogenase Through Subunit Splicing Promoted by Heterodimerization of Leucine Zipper Motifs

Peptide Bond Formation Between the Hetrosubunits of ω-Transaminase, Alanine Dehydrogenase, and Formate Dehydrogenase Through Subunit Splicing Promoted by Heterodimerization of Leucine Zipper Motifs

For the multimeric enzymes R-ω-transaminase (RTA), alanine dehydrogenase (AlaDH), and formate dehydrogenase (FDH), peptide bond formation between the hetrosubunits has been achieved by the intein-mediated in vivo subunit splicing. The subunit ligation is triggered by the heterodimerization of an arg...

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Bibliographic Details
Main Authors: Rong Li, Yao Chen, Kun Du, Wei Feng
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-06-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
R-ω-transaminase
alanine dehydrogenase
formate dehydrogenase
hetrosubunit splicing
leucine zipper motifs
Online Access:https://www.frontiersin.org/article/10.3389/fbioe.2020.00686/full

Internet

https://www.frontiersin.org/article/10.3389/fbioe.2020.00686/full

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