Expression, purification, and characterization of pyruvate kinase from Mycobacterium tuberculosis: A key allosteric regulatory enzyme

Expression, purification, and characterization of pyruvate kinase from Mycobacterium tuberculosis: A key allosteric regulatory enzyme

Background: The current research aims to isolate pyruvate kinase (Pyk) gene from Mycobacterium tuberculosis and expression of the gene (Rv1617) to obtain a purified enzyme. The enzyme activity and secondary structural features were assessed through biochemical assays and circular dichroism (CD) spec...

Full description

Bibliographic Details
Main Authors: Pavan Gollapali, Manjunath Chavdi, S J Sudharshan, Manjunatha Hanumanthappa
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2018-01-01
Series:International Journal of Mycobacteriology
Subjects:
Allosteric regulation
bacterial metabolism
circular dichroism
Mycobacterium tuberculosis
pathogenesis
pyruvate kinase
Online Access:http://www.ijmyco.org/article.asp?issn=2212-5531;year=2018;volume=7;issue=4;spage=368;epage=374;aulast=Gollapali

Internet

http://www.ijmyco.org/article.asp?issn=2212-5531;year=2018;volume=7;issue=4;spage=368;epage=374;aulast=Gollapali

Similar Items