Improved Protocol for the Production of the Low-Expression Eukaryotic Membrane Protein Human Aquaporin 2 in <i>Pichia pastoris</i> for Solid-State NMR

Improved Protocol for the Production of the Low-Expression Eukaryotic Membrane Protein Human Aquaporin 2 in <i>Pichia pastoris</i> for Solid-State NMR

Solid-state nuclear magnetic resonance (SSNMR) is a powerful biophysical technique for studies of membrane proteins; it requires the incorporation of isotopic labels into the sample. This is usually accomplished through over-expression of the protein of interest in a prokaryotic or eukaryotic host i...

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Bibliographic Details
Main Authors: Rachel Munro, Jeffrey de Vlugt, Vladimir Ladizhansky, Leonid S. Brown
Format: Article
Language:English
Published: MDPI AG 2020-03-01
Series:Biomolecules
Subjects:
biosynthetic isotope labeling
aquaporins
<i>pichia pastoris</i>
solid-state nmr
membrane proteins
post-translational modification
Online Access:https://www.mdpi.com/2218-273X/10/3/434

Internet

https://www.mdpi.com/2218-273X/10/3/434

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