Improved Protocol for the Production of the Low-Expression Eukaryotic Membrane Protein Human Aquaporin 2 in <i>Pichia pastoris</i> for Solid-State NMR
Solid-state nuclear magnetic resonance (SSNMR) is a powerful biophysical technique for studies of membrane proteins; it requires the incorporation of isotopic labels into the sample. This is usually accomplished through over-expression of the protein of interest in a prokaryotic or eukaryotic host i...
Main Authors: | Rachel Munro, Jeffrey de Vlugt, Vladimir Ladizhansky, Leonid S. Brown |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-03-01
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Series: | Biomolecules |
Subjects: | |
Online Access: | https://www.mdpi.com/2218-273X/10/3/434 |
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