Improving the Thermostability of Rhizopus chinensis Lipase Through Site-Directed Mutagenesis Based on B-Factor Analysis

In order to improve the thermostability of lipases derived from Rhizopus chinensis, we identified lipase (Lipr27RCL) mutagenesis sites that were associated with enhanced flexibility based upon B-factor analysis and multiple sequence alignment. We found that two mutated isoforms (Lipr27RCL-K64N and L...

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Main Authors: Zhanbao Jiang, Chengbo Zhang, Minyuan Tang, Bo Xu, Lili Wang, Wen Qian, Jiandong He, Zhihong Zhao, Qian Wu, Yuelin Mu, Junmei Ding, Rui Zhang, Zunxi Huang, Nanyu Han
Format: Article
Published: Frontiers Media S.A. 2020-03-01
Series:Frontiers in Microbiology
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