Improving the Thermostability of Rhizopus chinensis Lipase Through Site-Directed Mutagenesis Based on B-Factor Analysis
In order to improve the thermostability of lipases derived from Rhizopus chinensis, we identified lipase (Lipr27RCL) mutagenesis sites that were associated with enhanced flexibility based upon B-factor analysis and multiple sequence alignment. We found that two mutated isoforms (Lipr27RCL-K64N and L...
Main Authors: | Zhanbao Jiang, Chengbo Zhang, Minyuan Tang, Bo Xu, Lili Wang, Wen Qian, Jiandong He, Zhihong Zhao, Qian Wu, Yuelin Mu, Junmei Ding, Rui Zhang, Zunxi Huang, Nanyu Han |
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Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2020-03-01
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Series: | Frontiers in Microbiology |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.00346/full |
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