Semi-automated, occupationally safe immunofluorescence microtip sensor for rapid detection of Mycobacterium cells in sputum.

An occupationally safe (biosafe) sputum liquefaction protocol was developed for use with a semi-automated antibody-based microtip immunofluorescence sensor. The protocol effectively liquefied sputum and inactivated microorganisms including Mycobacterium tuberculosis, while preserving the antibody-bi...

Full description

Bibliographic Details
Main Authors: Shinnosuke Inoue, Annie L Becker, Jong-Hoon Kim, Zhiquan Shu, Scott D Soelberg, Kris M Weigel, Morgan Hiraiwa, Andrew Cairns, Hyun-Boo Lee, Clement E Furlong, Kieseok Oh, Kyong-Hoon Lee, Dayong Gao, Jae-Hyun Chung, Gerard A Cangelosi
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3899086?pdf=render