Structural properties of MHC class II ligands, implications for the prediction of MHC class II epitopes.
Major Histocompatibility class II (MHC-II) molecules sample peptides from the extracellular space allowing the immune system to detect the presence of foreign microbes from this compartment. Prediction of MHC class II ligands is complicated by the open binding cleft of the MHC class II molecule, all...
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doaj-47c8945066e2451c86dda1b7162bfc4a2020-11-25T00:23:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-12-01512e1587710.1371/journal.pone.0015877Structural properties of MHC class II ligands, implications for the prediction of MHC class II epitopes.Kasper Winther JørgensenSøren BuusMorten NielsenMajor Histocompatibility class II (MHC-II) molecules sample peptides from the extracellular space allowing the immune system to detect the presence of foreign microbes from this compartment. Prediction of MHC class II ligands is complicated by the open binding cleft of the MHC class II molecule, allowing binding of peptides extending out of the binding groove. Furthermore, only a few HLA-DR alleles have been characterized with a sufficient number of peptides (100-200 peptides per allele) to derive accurate description of their binding motif. Little work has been performed characterizing structural properties of MHC class II ligands. Here, we perform one such large-scale analysis. A large set of SYFPEITHI MHC class II ligands covering more than 20 different HLA-DR molecules was analyzed in terms of their secondary structure and surface exposure characteristics in the context of the native structure of the corresponding source protein. We demonstrated that MHC class II ligands are significantly more exposed and have significantly more coil content than other peptides in the same protein with similar predicted binding affinity. We next exploited this observation to derive an improved prediction method for MHC class II ligands by integrating prediction of MHC- peptide binding with prediction of surface exposure and protein secondary structure. This combined prediction method was shown to significantly outperform the state-of-the-art MHC class II peptide binding prediction method when used to identify MHC class II ligands. We also tried to integrate N- and O-glycosylation in our prediction methods but this additional information was found not to improve prediction performance. In summary, these findings strongly suggest that local structural properties influence antigen processing and/or the accessibility of peptides to the MHC class II molecule.http://europepmc.org/articles/PMC3012731?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kasper Winther Jørgensen Søren Buus Morten Nielsen |
spellingShingle |
Kasper Winther Jørgensen Søren Buus Morten Nielsen Structural properties of MHC class II ligands, implications for the prediction of MHC class II epitopes. PLoS ONE |
author_facet |
Kasper Winther Jørgensen Søren Buus Morten Nielsen |
author_sort |
Kasper Winther Jørgensen |
title |
Structural properties of MHC class II ligands, implications for the prediction of MHC class II epitopes. |
title_short |
Structural properties of MHC class II ligands, implications for the prediction of MHC class II epitopes. |
title_full |
Structural properties of MHC class II ligands, implications for the prediction of MHC class II epitopes. |
title_fullStr |
Structural properties of MHC class II ligands, implications for the prediction of MHC class II epitopes. |
title_full_unstemmed |
Structural properties of MHC class II ligands, implications for the prediction of MHC class II epitopes. |
title_sort |
structural properties of mhc class ii ligands, implications for the prediction of mhc class ii epitopes. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2010-12-01 |
description |
Major Histocompatibility class II (MHC-II) molecules sample peptides from the extracellular space allowing the immune system to detect the presence of foreign microbes from this compartment. Prediction of MHC class II ligands is complicated by the open binding cleft of the MHC class II molecule, allowing binding of peptides extending out of the binding groove. Furthermore, only a few HLA-DR alleles have been characterized with a sufficient number of peptides (100-200 peptides per allele) to derive accurate description of their binding motif. Little work has been performed characterizing structural properties of MHC class II ligands. Here, we perform one such large-scale analysis. A large set of SYFPEITHI MHC class II ligands covering more than 20 different HLA-DR molecules was analyzed in terms of their secondary structure and surface exposure characteristics in the context of the native structure of the corresponding source protein. We demonstrated that MHC class II ligands are significantly more exposed and have significantly more coil content than other peptides in the same protein with similar predicted binding affinity. We next exploited this observation to derive an improved prediction method for MHC class II ligands by integrating prediction of MHC- peptide binding with prediction of surface exposure and protein secondary structure. This combined prediction method was shown to significantly outperform the state-of-the-art MHC class II peptide binding prediction method when used to identify MHC class II ligands. We also tried to integrate N- and O-glycosylation in our prediction methods but this additional information was found not to improve prediction performance. In summary, these findings strongly suggest that local structural properties influence antigen processing and/or the accessibility of peptides to the MHC class II molecule. |
url |
http://europepmc.org/articles/PMC3012731?pdf=render |
work_keys_str_mv |
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