Nanopore long-read RNAseq reveals widespread transcriptional variation among the surface receptors of individual B cells

Nanopore long-read RNAseq reveals widespread transcriptional variation among the surface receptors of individual B cells

Short-read RNA-seq is limited in its ability to resolve complex transcript isoforms since it cannot sequence full-length cDNA. Here the authors use Oxford Nanopore MinION and their Mandalorion analysis pipeline to measure complex isoforms in B1a cells.

Bibliographic Details
Main Authors: Ashley Byrne, Anna E. Beaudin, Hugh E. Olsen, Miten Jain, Charles Cole, Theron Palmer, Rebecca M. DuBois, E. Camilla Forsberg, Mark Akeson, Christopher Vollmers
Format: Article
Language:English
Published: Nature Publishing Group 2017-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/ncomms16027

Internet

https://doi.org/10.1038/ncomms16027

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