Nanopore long-read RNAseq reveals widespread transcriptional variation among the surface receptors of individual B cells
Short-read RNA-seq is limited in its ability to resolve complex transcript isoforms since it cannot sequence full-length cDNA. Here the authors use Oxford Nanopore MinION and their Mandalorion analysis pipeline to measure complex isoforms in B1a cells.
Main Authors: | Ashley Byrne, Anna E. Beaudin, Hugh E. Olsen, Miten Jain, Charles Cole, Theron Palmer, Rebecca M. DuBois, E. Camilla Forsberg, Mark Akeson, Christopher Vollmers |
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Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2017-07-01
|
Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/ncomms16027 |
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