Physical Extraction and Fast Protein Liquid Chromatography for Purifying Flagella Filament From Uropathogenic Escherichia coli for Immune Assay

Physical Extraction and Fast Protein Liquid Chromatography for Purifying Flagella Filament From Uropathogenic Escherichia coli for Immune Assay

Flagella are expressed on the surface of a wide range of bacteria, conferring motility and contributing to virulence and innate immune stimulation. Host-pathogen interaction studies of the roles of flagella in infection, including due to uropathogenic Escherichia coli (UPEC), have used various metho...

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Bibliographic Details
Main Authors: Dhruba Acharya, Matthew J. Sullivan, Benjamin L. Duell, Tanguy Eveno, Mark A. Schembri, Glen C. Ulett
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-04-01
Series:Frontiers in Cellular and Infection Microbiology
Subjects:
uropathogenic Escherichia coli
UPEC
Flagella
FliC flagellin
fast protein liquid chromatography
FPLC
Online Access:https://www.frontiersin.org/article/10.3389/fcimb.2019.00118/full

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https://www.frontiersin.org/article/10.3389/fcimb.2019.00118/full

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