Activity Improvement and Vital Amino Acid Identification on the Marine-Derived Quorum Quenching Enzyme MomL by Protein Engineering

Activity Improvement and Vital Amino Acid Identification on the Marine-Derived Quorum Quenching Enzyme MomL by Protein Engineering

MomL is a marine-derived quorum-quenching (QQ) lactonase which can degrade various <i>N</i>-acyl homoserine lactones (AHLs). Intentional modification of MomL may lead to a highly efficient QQ enzyme with broad application potential. In this study, we used a rapid and efficient method com...

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Bibliographic Details
Main Authors: Jiayi Wang, Jing Lin, Yunhui Zhang, Jingjing Zhang, Tao Feng, Hui Li, Xianghong Wang, Qingyang Sun, Xiaohua Zhang, Yan Wang
Format: Article
Language:English
Published: MDPI AG 2019-05-01
Series:Marine Drugs
Subjects:
quorum quenching enzyme
error prone PCR
high-throughput screening
site-directed mutagenesis
catalytic ability
<i>Pectobacterium carotovorum</i> subsp. <i>carotovorum (Pcc)</i>
Online Access:https://www.mdpi.com/1660-3397/17/5/300

Internet

https://www.mdpi.com/1660-3397/17/5/300

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