BurkDiff: a real-time PCR allelic discrimination assay for Burkholderia pseudomallei and B. mallei.
A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2010-11-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC2980470?pdf=render |