BurkDiff: a real-time PCR allelic discrimination assay for Burkholderia pseudomallei and B. mallei.

A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of...

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Main Authors:	Jolene R Bowers, David M Engelthaler, Jennifer L Ginther, Talima Pearson, Sharon J Peacock, Apichai Tuanyok, David M Wagner, Bart J Currie, Paul S Keim
Format:	Article
Language:	English
Published:	Public Library of Science (PLoS) 2010-11-01
Series:	PLoS ONE
Online Access:	http://europepmc.org/articles/PMC2980470?pdf=render

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spelling	doaj-48e1e81b21f04395a33c0ab52c8289702020-11-24T20:46:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-11-01511e1541310.1371/journal.pone.0015413BurkDiff: a real-time PCR allelic discrimination assay for Burkholderia pseudomallei and B. mallei.Jolene R BowersDavid M EngelthalerJennifer L GintherTalima PearsonSharon J PeacockApichai TuanyokDavid M WagnerBart J CurriePaul S KeimA real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei.http://europepmc.org/articles/PMC2980470?pdf=render
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Jolene R Bowers David M Engelthaler Jennifer L Ginther Talima Pearson Sharon J Peacock Apichai Tuanyok David M Wagner Bart J Currie Paul S Keim
spellingShingle	Jolene R Bowers David M Engelthaler Jennifer L Ginther Talima Pearson Sharon J Peacock Apichai Tuanyok David M Wagner Bart J Currie Paul S Keim BurkDiff: a real-time PCR allelic discrimination assay for Burkholderia pseudomallei and B. mallei. PLoS ONE
author_facet	Jolene R Bowers David M Engelthaler Jennifer L Ginther Talima Pearson Sharon J Peacock Apichai Tuanyok David M Wagner Bart J Currie Paul S Keim
author_sort	Jolene R Bowers
title	BurkDiff: a real-time PCR allelic discrimination assay for Burkholderia pseudomallei and B. mallei.
title_short	BurkDiff: a real-time PCR allelic discrimination assay for Burkholderia pseudomallei and B. mallei.
title_full	BurkDiff: a real-time PCR allelic discrimination assay for Burkholderia pseudomallei and B. mallei.
title_fullStr	BurkDiff: a real-time PCR allelic discrimination assay for Burkholderia pseudomallei and B. mallei.
title_full_unstemmed	BurkDiff: a real-time PCR allelic discrimination assay for Burkholderia pseudomallei and B. mallei.
title_sort	burkdiff: a real-time pcr allelic discrimination assay for burkholderia pseudomallei and b. mallei.
publisher	Public Library of Science (PLoS)
series	PLoS ONE
issn	1932-6203
publishDate	2010-11-01
description	A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei.
url	http://europepmc.org/articles/PMC2980470?pdf=render
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BurkDiff: a real-time PCR allelic discrimination assay for Burkholderia pseudomallei and B. mallei.

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