BurkDiff: a real-time PCR allelic discrimination assay for Burkholderia pseudomallei and B. mallei.

BurkDiff: a real-time PCR allelic discrimination assay for Burkholderia pseudomallei and B. mallei.

A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of...

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Bibliographic Details
Main Authors: Jolene R Bowers, David M Engelthaler, Jennifer L Ginther, Talima Pearson, Sharon J Peacock, Apichai Tuanyok, David M Wagner, Bart J Currie, Paul S Keim
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2010-11-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC2980470?pdf=render

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