Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids

Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids

Gene knock-in techniques have rapidly evolved in recent years, along with the development and maturation of genome editing technology using programmable nucleases. We recently reported a novel strategy for microhomology-mediated end-joining-dependent integration of donor DNA by using TALEN or CRISPR...

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Bibliographic Details
Main Authors: Tetsushi Sakuma, Mitsumasa Takenaga, Yoshinori Kawabe, Takahiro Nakamura, Masamichi Kamihira, Takashi Yamamoto
Format: Article
Language:English
Published: MDPI AG 2015-10-01
Series:International Journal of Molecular Sciences
Subjects:
TALEN
gene knock-in
CHO cells
microhomology-mediated end-joining
Online Access:http://www.mdpi.com/1422-0067/16/10/23849

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http://www.mdpi.com/1422-0067/16/10/23849

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