Application of Aptamers Improves CRISPR-Based Live Imaging of Plant Telomeres

Development of live imaging techniques for providing information how chromatin is organized in living cells is pivotal to decipher the regulation of biological processes. Here, we demonstrate the improvement of a live imaging technique based on CRISPR/Cas9. In this approach, the sgRNA scaffold is fu...

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Bibliographic Details
Main Authors: Solmaz Khosravi, Patrick Schindele, Evgeny Gladilin, Frank Dunemann, Twan Rutten, Holger Puchta, Andreas Houben
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-08-01
Series:Frontiers in Plant Science
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fpls.2020.01254/full