Protocol for Quantifying Zinc Flux in Cultured Cells using Fluorescent Indicators

Summary: Zinc (Zn2+) plays a vital role in the functioning of the cell. Cells have influx and efflux zinc transporters to regulate the levels of Zn2+ in the cytoplasm and organellar compartments to maintain homeostasis. We present a protocol to measure changes in cellular zinc concentrations using e...

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Bibliographic Details
Main Authors: Saima Ali, Math P. Cuajungco
Format: Article
Language:English
Published: Elsevier 2020-09-01
Series:STAR Protocols
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672030037X