Immune Effects of the Nitrated Food Allergen Beta-Lactoglobulin in an Experimental Food Allergy Model
Food proteins may get nitrated by various exogenous or endogenous mechanisms. As individuals might get recurrently exposed to nitrated proteins via daily diet, we aimed to investigate the effect of repeatedly ingested nitrated food proteins on the subsequent immune response in non-allergic and aller...
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doaj-49744b5f98e645c5819235fdc5b4dbd82020-11-25T01:54:57ZengMDPI AGNutrients2072-66432019-10-011110246310.3390/nu11102463nu11102463Immune Effects of the Nitrated Food Allergen Beta-Lactoglobulin in an Experimental Food Allergy ModelAnna S. Ondracek0Denise Heiden1Gertie J. Oostingh2Elisabeth Fuerst3Judit Fazekas-Singer4Cornelia Bergmayr5Johanna Rohrhofer6Erika Jensen-Jarolim7Albert Duschl8Eva Untersmayr9Institute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, AustriaInstitute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, AustriaBiomedical Sciences, Salzburg University of Applied Sciences, 5412 Puch/Salzburg, AustriaInstitute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, AustriaInstitute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, AustriaInstitute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, AustriaInstitute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, AustriaInstitute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Biosciences, University of Salzburg, 5020 Salzburg, AustriaInstitute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, AustriaFood proteins may get nitrated by various exogenous or endogenous mechanisms. As individuals might get recurrently exposed to nitrated proteins via daily diet, we aimed to investigate the effect of repeatedly ingested nitrated food proteins on the subsequent immune response in non-allergic and allergic mice using the milk allergen beta-lactoglobulin (BLG) as model food protein in a mouse model. Evaluating the presence of nitrated proteins in food, we could detect 3-nitrotyrosine (3-NT) in extracts of different foods and in stomach content extracts of non-allergic mice under physiological conditions. Chemically nitrated BLG (BLGn) exhibited enhanced susceptibility to degradation in simulated gastric fluid experiments compared to untreated BLG (BLGu). Gavage of BLGn to non-allergic animals increased interferon-γ and interleukin-10 release of stimulated spleen cells and led to the formation of BLG-specific serum IgA. Allergic mice receiving three oral gavages of BLGn had higher levels of mouse mast cell protease-1 (mMCP-1) compared to allergic mice receiving BLGu. Regardless of the preceding immune status, non-allergic or allergic, repeatedly ingested nitrated food proteins seem to considerably influence the subsequent immune response.https://www.mdpi.com/2072-6643/11/10/2463beta-lactoglobulinfood allergynitration |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anna S. Ondracek Denise Heiden Gertie J. Oostingh Elisabeth Fuerst Judit Fazekas-Singer Cornelia Bergmayr Johanna Rohrhofer Erika Jensen-Jarolim Albert Duschl Eva Untersmayr |
spellingShingle |
Anna S. Ondracek Denise Heiden Gertie J. Oostingh Elisabeth Fuerst Judit Fazekas-Singer Cornelia Bergmayr Johanna Rohrhofer Erika Jensen-Jarolim Albert Duschl Eva Untersmayr Immune Effects of the Nitrated Food Allergen Beta-Lactoglobulin in an Experimental Food Allergy Model Nutrients beta-lactoglobulin food allergy nitration |
author_facet |
Anna S. Ondracek Denise Heiden Gertie J. Oostingh Elisabeth Fuerst Judit Fazekas-Singer Cornelia Bergmayr Johanna Rohrhofer Erika Jensen-Jarolim Albert Duschl Eva Untersmayr |
author_sort |
Anna S. Ondracek |
title |
Immune Effects of the Nitrated Food Allergen Beta-Lactoglobulin in an Experimental Food Allergy Model |
title_short |
Immune Effects of the Nitrated Food Allergen Beta-Lactoglobulin in an Experimental Food Allergy Model |
title_full |
Immune Effects of the Nitrated Food Allergen Beta-Lactoglobulin in an Experimental Food Allergy Model |
title_fullStr |
Immune Effects of the Nitrated Food Allergen Beta-Lactoglobulin in an Experimental Food Allergy Model |
title_full_unstemmed |
Immune Effects of the Nitrated Food Allergen Beta-Lactoglobulin in an Experimental Food Allergy Model |
title_sort |
immune effects of the nitrated food allergen beta-lactoglobulin in an experimental food allergy model |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2019-10-01 |
description |
Food proteins may get nitrated by various exogenous or endogenous mechanisms. As individuals might get recurrently exposed to nitrated proteins via daily diet, we aimed to investigate the effect of repeatedly ingested nitrated food proteins on the subsequent immune response in non-allergic and allergic mice using the milk allergen beta-lactoglobulin (BLG) as model food protein in a mouse model. Evaluating the presence of nitrated proteins in food, we could detect 3-nitrotyrosine (3-NT) in extracts of different foods and in stomach content extracts of non-allergic mice under physiological conditions. Chemically nitrated BLG (BLGn) exhibited enhanced susceptibility to degradation in simulated gastric fluid experiments compared to untreated BLG (BLGu). Gavage of BLGn to non-allergic animals increased interferon-γ and interleukin-10 release of stimulated spleen cells and led to the formation of BLG-specific serum IgA. Allergic mice receiving three oral gavages of BLGn had higher levels of mouse mast cell protease-1 (mMCP-1) compared to allergic mice receiving BLGu. Regardless of the preceding immune status, non-allergic or allergic, repeatedly ingested nitrated food proteins seem to considerably influence the subsequent immune response. |
topic |
beta-lactoglobulin food allergy nitration |
url |
https://www.mdpi.com/2072-6643/11/10/2463 |
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