Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis

Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis

Abstract Prolactin (PRL) is a hormone produced by the pituitary gland with innumerable functions, such as lactation, reproduction, osmotic and immune regulation. The present work describes the synthesis of hPRL in human embryonic kidney (HEK293) cells, transiently transfected with the pcDNA-3.4-TOPO...

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Bibliographic Details
Main Authors: Felipe D. Silva, João E. Oliveira, Renan P. Freire, Miriam F. Suzuki, Carlos R. Soares, Paolo Bartolini
Format: Article
Language:English
Published: SpringerOpen 2019-08-01
Series:AMB Express
Subjects:
N-Glycoprofiling
N-Glycans
Human prolactin
HEK293 cell
MALDI–TOF-MS
Online Access:http://link.springer.com/article/10.1186/s13568-019-0856-8

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http://link.springer.com/article/10.1186/s13568-019-0856-8

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