Quantification of adipocyte numbers in transgenic mice via the Cre-LoxP recombination sites

Quantification of adipocyte numbers in transgenic mice via the Cre-LoxP recombination sites

Summary: This protocol describes a method to assess adipocyte numbers within a specific depot based on their inducible genomic label. By extracting DNA from a complete adipose tissue depot stemming from two transgenic mouse lines (Adipoq-CreERT2 x ROSA26-tdRFP and Ucp1-CreERT2 x ROSA26-tdRFP), the n...

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Bibliographic Details
Main Authors: Caroline Moser, Leon G. Straub, Yael Rachamin, Christian Wolfrum
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Cell culture
Cell isolation
Cell-based Assays
Cell separation/fractionation
Metabolism
Molecular Biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721004676

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http://www.sciencedirect.com/science/article/pii/S2666166721004676

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