A comprehensive method for extraction and quantitative analysis of sterols and secosteroids from human plasma

A comprehensive method for extraction and quantitative analysis of sterols and secosteroids from human plasma

We describe the development of a method for the extraction and analysis of 62 sterols, oxysterols, and secosteroids from human plasma using a combination of HPLC-MS and GC-MS. Deuterated standards are added to 200 μl of human plasma. Bulk lipids are extracted with methanol:dichloromethane, the sampl...

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Bibliographic Details
Main Authors: Jeffrey G. McDonald, Daniel D. Smith, Ashlee R. Stiles, David W. Russell
Format: Article
Language:English
Published: Elsevier 2012-07-01
Series:Journal of Lipid Research
Subjects:
cholesterol/biosynthesis
cholesterol/metabolism
lipidomics
mass spectrometry
oxysterols
steroid hormones
Online Access:http://www.sciencedirect.com/science/article/pii/S002222752034517X

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http://www.sciencedirect.com/science/article/pii/S002222752034517X

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