A comprehensive method for extraction and quantitative analysis of sterols and secosteroids from human plasma
We describe the development of a method for the extraction and analysis of 62 sterols, oxysterols, and secosteroids from human plasma using a combination of HPLC-MS and GC-MS. Deuterated standards are added to 200 μl of human plasma. Bulk lipids are extracted with methanol:dichloromethane, the sampl...
Main Authors: | Jeffrey G. McDonald, Daniel D. Smith, Ashlee R. Stiles, David W. Russell |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2012-07-01
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Series: | Journal of Lipid Research |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S002222752034517X |
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