Preferential Identification of Agonistic OX40 Antibodies by Using Cell Lysate to Pan Natively Paired, Humanized Mouse-Derived Yeast Surface Display Libraries

Preferential Identification of Agonistic OX40 Antibodies by Using Cell Lysate to Pan Natively Paired, Humanized Mouse-Derived Yeast Surface Display Libraries

To discover therapeutically relevant antibody candidates, many groups use mouse immunization followed by hybridoma generation or B cell screening. One modern approach is to screen B cells by generating natively paired single chain variable fragment (scFv) display libraries in yeast. Such methods typ...

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Bibliographic Details
Main Authors: Angélica V. Medina-Cucurella, Rena A. Mizrahi, Michael A. Asensio, Robert C. Edgar, Jackson Leong, Renee Leong, Yoong Wearn Lim, Ayla Nelson, Ariel R. Niedecken, Jan Fredrik Simons, Matthew J. Spindler, Kacy Stadtmiller, Nicholas Wayham, Adam S. Adler, David S. Johnson
Format: Article
Language:English
Published: MDPI AG 2019-02-01
Series:Antibodies
Subjects:
OX40
humanized mouse antibody repertoires
deep sequencing
yeast display
Online Access:https://www.mdpi.com/2073-4468/8/1/17

Internet

https://www.mdpi.com/2073-4468/8/1/17

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