Untargeted Metabolomics of Fermented Rice Using UHPLC Q-TOF MS/MS Reveals an Abundance of Potential Antihypertensive Compounds
Enzyme treatment and fermentation of cereals are known processes that enhance the release of bound bioactive compounds to make them available for bioactivity. In this study, we tested the angiotensin converting enzyme (ACE) inhibitory ability of destarched rice, Prozyme 2000p treated destarched rice...
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doaj-4a1026d21a904dbfa8dee2c1b29493a72020-11-25T03:28:58ZengMDPI AGFoods2304-81582020-07-0191007100710.3390/foods9081007Untargeted Metabolomics of Fermented Rice Using UHPLC Q-TOF MS/MS Reveals an Abundance of Potential Antihypertensive CompoundsEric Banan-Mwine Daliri0Fred Kwame Ofosu1Ramachandran Chelliah2Joong-Hark Kim3Jong-Rae Kim4Daesang Yoo5Deog-Hwan Oh6Department of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, Gangwon-do, KoreaDepartment of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, Gangwon-do, KoreaDepartment of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, Gangwon-do, KoreaDepartment of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon 24341, Gangwon-do, KoreaDepartment of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, Gangwon-do, KoreaDepartment of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, Gangwon-do, KoreaDepartment of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, Gangwon-do, KoreaEnzyme treatment and fermentation of cereals are known processes that enhance the release of bound bioactive compounds to make them available for bioactivity. In this study, we tested the angiotensin converting enzyme (ACE) inhibitory ability of destarched rice, Prozyme 2000p treated destarched rice (DP), and fermented DP samples. Prozyme 2000p treatment increased the ACE inhibitory ability from 15 ± 5% to 45 ± 3%. Fermentation of the Prozyme 2000p treated samples with <i>Enterococcus faecium</i> EBD1 significantly increased the ACE inhibitory ability to 75 ± 5%, while captopril showed an ACE inhibition of 92 ± 4%. An untargeted metabolomics approach using Ultra-high-performance liquid tandem chromatography quadrupole time of flight mass spectrometry revealed the abundance of vitamins, phenolic compounds, antioxidant peptides, DPP IV inhibitory peptides, and antihypertensive peptides in the fermented samples which may account for its strong ACE inhibition. Although fermented DP had decreased fatty acid levels, the amount of essential amino acid improved drastically compared to destarched rice. Our results show that fermenting Prozyme-treated destarched rice with <i>Enterococcus faecium</i> EBD1 generates abundant bioactive compounds necessary for developing antihypertensive functional foods.https://www.mdpi.com/2304-8158/9/8/1007bioactive peptideshypertensionphenolic compoundsfunctional food |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Eric Banan-Mwine Daliri Fred Kwame Ofosu Ramachandran Chelliah Joong-Hark Kim Jong-Rae Kim Daesang Yoo Deog-Hwan Oh |
spellingShingle |
Eric Banan-Mwine Daliri Fred Kwame Ofosu Ramachandran Chelliah Joong-Hark Kim Jong-Rae Kim Daesang Yoo Deog-Hwan Oh Untargeted Metabolomics of Fermented Rice Using UHPLC Q-TOF MS/MS Reveals an Abundance of Potential Antihypertensive Compounds Foods bioactive peptides hypertension phenolic compounds functional food |
author_facet |
Eric Banan-Mwine Daliri Fred Kwame Ofosu Ramachandran Chelliah Joong-Hark Kim Jong-Rae Kim Daesang Yoo Deog-Hwan Oh |
author_sort |
Eric Banan-Mwine Daliri |
title |
Untargeted Metabolomics of Fermented Rice Using UHPLC Q-TOF MS/MS Reveals an Abundance of Potential Antihypertensive Compounds |
title_short |
Untargeted Metabolomics of Fermented Rice Using UHPLC Q-TOF MS/MS Reveals an Abundance of Potential Antihypertensive Compounds |
title_full |
Untargeted Metabolomics of Fermented Rice Using UHPLC Q-TOF MS/MS Reveals an Abundance of Potential Antihypertensive Compounds |
title_fullStr |
Untargeted Metabolomics of Fermented Rice Using UHPLC Q-TOF MS/MS Reveals an Abundance of Potential Antihypertensive Compounds |
title_full_unstemmed |
Untargeted Metabolomics of Fermented Rice Using UHPLC Q-TOF MS/MS Reveals an Abundance of Potential Antihypertensive Compounds |
title_sort |
untargeted metabolomics of fermented rice using uhplc q-tof ms/ms reveals an abundance of potential antihypertensive compounds |
publisher |
MDPI AG |
series |
Foods |
issn |
2304-8158 |
publishDate |
2020-07-01 |
description |
Enzyme treatment and fermentation of cereals are known processes that enhance the release of bound bioactive compounds to make them available for bioactivity. In this study, we tested the angiotensin converting enzyme (ACE) inhibitory ability of destarched rice, Prozyme 2000p treated destarched rice (DP), and fermented DP samples. Prozyme 2000p treatment increased the ACE inhibitory ability from 15 ± 5% to 45 ± 3%. Fermentation of the Prozyme 2000p treated samples with <i>Enterococcus faecium</i> EBD1 significantly increased the ACE inhibitory ability to 75 ± 5%, while captopril showed an ACE inhibition of 92 ± 4%. An untargeted metabolomics approach using Ultra-high-performance liquid tandem chromatography quadrupole time of flight mass spectrometry revealed the abundance of vitamins, phenolic compounds, antioxidant peptides, DPP IV inhibitory peptides, and antihypertensive peptides in the fermented samples which may account for its strong ACE inhibition. Although fermented DP had decreased fatty acid levels, the amount of essential amino acid improved drastically compared to destarched rice. Our results show that fermenting Prozyme-treated destarched rice with <i>Enterococcus faecium</i> EBD1 generates abundant bioactive compounds necessary for developing antihypertensive functional foods. |
topic |
bioactive peptides hypertension phenolic compounds functional food |
url |
https://www.mdpi.com/2304-8158/9/8/1007 |
work_keys_str_mv |
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