A rapid calcium precipitation method of recovering large amounts of highly pure hepatocyte rough endoplasmic reticulum

A rapid calcium precipitation method of recovering large amounts of highly pure hepatocyte rough endoplasmic reticulum

We sought a rapid and non-ultracentrifugal method of recovering large amounts of highly pure rough endoplasmic reticulum (RER) membranes from livers. By substantially modifying a 20-year-old calcium precipitation technique, we obtained a RER fraction from rat liver and established its high degree of...

Full description

Bibliographic Details
Main Authors: Robert L. Hamilton, Anne Moorehouse, Steven R. Lear, Jinny S. Wong, Sandra K. Erickson
Format: Article
Language:English
Published: Elsevier 1999-06-01
Series:Journal of Lipid Research
Subjects:
apolipoprotein-B
microsomal triglyceride transfer protein
acyl coenzyme A:diacylglycerol acyltransferase
acyl coenzyme A:cholesterol acyltransferase
electron microscopy
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520335185

Internet

http://www.sciencedirect.com/science/article/pii/S0022227520335185

Similar Items