Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones

Use of homologous recombination in yeast to create chimeric bovine viral diarrhea virus cDNA clones

Abstract The open reading frame of a Brazilian bovine viral diarrhea virus (BVDV) strain, IBSP4ncp, was recombined with the untranslated regions of the reference NADL strain by homologous recombination in Saccharomyces cerevisiae, resulting in chimeric full-length cDNA clones of BVDV (chi-NADL/IBSP4...

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Bibliographic Details
Main Authors: Sandra Arenhart, José Valter Joaquim Silva Junior, Eduardo Furtado Flores, Rudi Weiblen, Laura Helena Vega Gonzales Gil
Format: Article
Language:English
Published: Sociedade Brasileira de Microbiologia
Series:Brazilian Journal of Microbiology
Subjects:
BVDV
Pestivirus
Reverse genetics
Infectious clone
Yeast homologous recombination
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400993&lng=en&tlng=en

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400993&lng=en&tlng=en

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