Catalytic Performance of a Class III Old Yellow Enzyme and Its Cysteine Variants

Catalytic Performance of a Class III Old Yellow Enzyme and Its Cysteine Variants

Class III old yellow enzymes (OYEs) contain a conserved cysteine in their active sites. To address the role of this cysteine in OYE-mediated asymmetric synthesis, we have studied the biocatalytic properties of OYERo2a from Rhodococcus opacus 1CP (WT) as well as its engineered variants C25A, C25S and...

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Bibliographic Details
Main Authors: Anika Scholtissek, Eric Gädke, Caroline E. Paul, Adrie H. Westphal, Willem J. H. van Berkel, Dirk Tischler
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-10-01
Series:Frontiers in Microbiology
Subjects:
biocatalysis
ene reductase
flavoprotein
inactivation
actinobacteria
protein engineering
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2018.02410/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2018.02410/full

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