Functional Characterization and Structural Analysis of NADH Oxidase Mutants from <em>Thermus thermophilus</em> HB27: Role of Residues 166, 174, and 194 in the Catalytic Properties and Thermostability

Functional Characterization and Structural Analysis of NADH Oxidase Mutants from <em>Thermus thermophilus</em> HB27: Role of Residues 166, 174, and 194 in the Catalytic Properties and Thermostability

The <i>Thermus thermophilus</i> strain HB27 NADH-oxidase (Tt27-NOX) catalyzes the oxidation of nicotinamide adenine dinucleotide (NAD(P)H) by reducing molecular oxygen to hydrogen peroxide in a two-electron transfer mechanism. Surprisingly, Tt27-NOX showed significant differences in cata...

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Bibliographic Details
Main Authors: Javier Rocha-Martin, Pedro A. Sánchez-Murcia, Fernando López-Gallego, Aurelio Hidalgo, José Berenguer, José M. Guisan
Format: Article
Language:English
Published: MDPI AG 2019-10-01
Series:Microorganisms
Subjects:
nadh oxidase
cofactor regeneration
hydrogen peroxide
dehydrogenase
nad<sup>+</sup>
extremophiles
Online Access:https://www.mdpi.com/2076-2607/7/11/515

Internet

https://www.mdpi.com/2076-2607/7/11/515

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