Molecular Analysis of Bacterial Community DNA in Sludge Undergoing Autothermal Thermophilic Aerobic Digestion (ATAD): Pitfalls and Improved Methodology to Enhance Diversity Recovery

Molecular Analysis of Bacterial Community DNA in Sludge Undergoing Autothermal Thermophilic Aerobic Digestion (ATAD): Pitfalls and Improved Methodology to Enhance Diversity Recovery

Molecular analysis of the bacterial community structure associated with sludge processed by autothermal thermophilic aerobic digestion (ATAD), was performed using a number of extraction and amplification procedures which differed in yield, integrity, ability to amplify extracted templates and specif...

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Bibliographic Details
Main Authors: Anna V. Piterina, John Bartlett, J. Tony Pembroke
Format: Article
Language:English
Published: MDPI AG 2010-03-01
Series:Diversity
Subjects:
ATAD
thermophilic sludge
DNA extraction
PCR optimisation
PCR inhibition
microbial community analysis
DGGE
clone library
Online Access:http://www.mdpi.com/1424-2818/2/4/505/

Internet

http://www.mdpi.com/1424-2818/2/4/505/

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