Inactivation of Latent HIV-1 Proviral DNA Using Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 Treatment and the Assessment of Off-Target Effects

Inactivation of Latent HIV-1 Proviral DNA Using Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 Treatment and the Assessment of Off-Target Effects

Viral DNA integrated in host cells is a major barrier to completely curing HIV-1. However, genome editing using the recently developed technique of clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 has the potential to eradicate HIV-1. The present study aimed to use a lentivira...

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Bibliographic Details
Main Authors: Yufan Xu, Xiaorong Peng, Yanghao Zheng, Changzhong Jin, Xiangyun Lu, Dating Han, Haijing Fu, Chaoyu Chen, Nanping Wu
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-05-01
Series:Frontiers in Microbiology
Subjects:
CRISPR/Cas9
HIV-1
dual-sgRNAs
off-target effect
genome editing
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2021.629153/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2021.629153/full

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