CD169 Defines Activated CD14+ Monocytes With Enhanced CD8+ T Cell Activation Capacity
Monocytes are antigen-presenting cells (APCs) that play diverse roles in promoting or regulating inflammatory responses, but their role in T cell stimulation is not well defined. In inflammatory conditions, monocytes frequently show increased expression of CD169/Siglec-1, a type-I interferon (IFN-I)...
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Frontiers Media S.A.
2021-07-01
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Series: | Frontiers in Immunology |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.697840/full |
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Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alsya J. Affandi Katarzyna Olesek Joanna Grabowska Maarten K. Nijen Twilhaar Ernesto Rodríguez Anno Saris Anno Saris Eline S. Zwart Eline S. Zwart Esther J. Nossent Esther J. Nossent Hakan Kalay Michael de Kok Geert Kazemier Johannes Stöckl Alfons J. M. van den Eertwegh Tanja D. de Gruijl Juan J. Garcia-Vallejo Gert Storm Gert Storm Gert Storm Yvette van Kooyk Joke M. M. den Haan |
spellingShingle |
Alsya J. Affandi Katarzyna Olesek Joanna Grabowska Maarten K. Nijen Twilhaar Ernesto Rodríguez Anno Saris Anno Saris Eline S. Zwart Eline S. Zwart Esther J. Nossent Esther J. Nossent Hakan Kalay Michael de Kok Geert Kazemier Johannes Stöckl Alfons J. M. van den Eertwegh Tanja D. de Gruijl Juan J. Garcia-Vallejo Gert Storm Gert Storm Gert Storm Yvette van Kooyk Joke M. M. den Haan CD169 Defines Activated CD14+ Monocytes With Enhanced CD8+ T Cell Activation Capacity Frontiers in Immunology monocyte CD169 antigen-presentation CD8+ T cell nanovaccine cancer |
author_facet |
Alsya J. Affandi Katarzyna Olesek Joanna Grabowska Maarten K. Nijen Twilhaar Ernesto Rodríguez Anno Saris Anno Saris Eline S. Zwart Eline S. Zwart Esther J. Nossent Esther J. Nossent Hakan Kalay Michael de Kok Geert Kazemier Johannes Stöckl Alfons J. M. van den Eertwegh Tanja D. de Gruijl Juan J. Garcia-Vallejo Gert Storm Gert Storm Gert Storm Yvette van Kooyk Joke M. M. den Haan |
author_sort |
Alsya J. Affandi |
title |
CD169 Defines Activated CD14+ Monocytes With Enhanced CD8+ T Cell Activation Capacity |
title_short |
CD169 Defines Activated CD14+ Monocytes With Enhanced CD8+ T Cell Activation Capacity |
title_full |
CD169 Defines Activated CD14+ Monocytes With Enhanced CD8+ T Cell Activation Capacity |
title_fullStr |
CD169 Defines Activated CD14+ Monocytes With Enhanced CD8+ T Cell Activation Capacity |
title_full_unstemmed |
CD169 Defines Activated CD14+ Monocytes With Enhanced CD8+ T Cell Activation Capacity |
title_sort |
cd169 defines activated cd14+ monocytes with enhanced cd8+ t cell activation capacity |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2021-07-01 |
description |
Monocytes are antigen-presenting cells (APCs) that play diverse roles in promoting or regulating inflammatory responses, but their role in T cell stimulation is not well defined. In inflammatory conditions, monocytes frequently show increased expression of CD169/Siglec-1, a type-I interferon (IFN-I)-regulated protein. However, little is known about the phenotype and function of these CD169+ monocytes. Here, we have investigated the phenotype of human CD169+ monocytes in different diseases, their capacity to activate CD8+ T cells, and the potential for a targeted-vaccination approach. Using spectral flow cytometry, we detected CD169 expression by CD14+ CD16- classical and CD14+ CD16+ intermediate monocytes and unbiased analysis showed that they were distinct from dendritic cells, including the recently described CD14-expressing DC3. CD169+ monocytes expressed higher levels of co-stimulatory and HLA molecules, suggesting an increased activation state. IFNα treatment highly upregulated CD169 expression on CD14+ monocytes and boosted their capacity to cross-present antigen to CD8+ T cells. Furthermore, we observed CD169+ monocytes in virally-infected patients, including in the blood and bronchoalveolar lavage fluid of COVID-19 patients, as well as in the blood of patients with different types of cancers. Finally, we evaluated two CD169-targeting nanovaccine platforms, antibody-based and liposome-based, and we showed that CD169+ monocytes efficiently presented tumor-associated peptides gp100 and WT1 to antigen-specific CD8+ T cells. In conclusion, our data indicate that CD169+ monocytes are activated monocytes with enhanced CD8+ T cell stimulatory capacity and that they emerge as an interesting target in nanovaccine strategies, because of their presence in health and different diseases. |
topic |
monocyte CD169 antigen-presentation CD8+ T cell nanovaccine cancer |
url |
https://www.frontiersin.org/articles/10.3389/fimmu.2021.697840/full |
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doaj-4b4b49035970432f8d4a489d0e951e6f2021-07-28T15:00:25ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-07-011210.3389/fimmu.2021.697840697840CD169 Defines Activated CD14+ Monocytes With Enhanced CD8+ T Cell Activation CapacityAlsya J. Affandi0Katarzyna Olesek1Joanna Grabowska2Maarten K. Nijen Twilhaar3Ernesto Rodríguez4Anno Saris5Anno Saris6Eline S. Zwart7Eline S. Zwart8Esther J. Nossent9Esther J. Nossent10Hakan Kalay11Michael de Kok12Geert Kazemier13Johannes Stöckl14Alfons J. M. van den Eertwegh15Tanja D. de Gruijl16Juan J. Garcia-Vallejo17Gert Storm18Gert Storm19Gert Storm20Yvette van Kooyk21Joke M. M. den Haan22Department of Molecular Cell Biology and Immunology, Cancer Center Amsterdam, Amsterdam Institute for Infection and Immunity, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam, NetherlandsDepartment of Molecular Cell Biology and Immunology, Cancer Center Amsterdam, Amsterdam Institute for Infection and Immunity, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam, NetherlandsDepartment of Molecular Cell Biology and Immunology, Cancer Center Amsterdam, Amsterdam Institute for Infection and Immunity, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam, NetherlandsDepartment of Molecular Cell Biology and Immunology, Cancer Center Amsterdam, Amsterdam Institute for Infection and Immunity, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam, NetherlandsDepartment of Molecular Cell Biology and Immunology, Cancer Center Amsterdam, Amsterdam Institute for Infection and Immunity, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam, NetherlandsCenter for Experimental and Molecular Medicine, Amsterdam UMC, Academic Medical Center, University of Amsterdam, Amsterdam, NetherlandsDepartment of Infectious Diseases, Leiden University Medical Center, Leiden, NetherlandsDepartment of Molecular Cell Biology and Immunology, Cancer Center Amsterdam, Amsterdam Institute for Infection and Immunity, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam, NetherlandsDepartment of Surgery, Cancer Center Amsterdam, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam, NetherlandsDepartment of Pulmonary Medicine, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam, NetherlandsAmsterdam Cardiovascular Sciences Research Institute, Amsterdam UMC, Amsterdam, NetherlandsDepartment of Molecular Cell Biology and Immunology, Cancer Center Amsterdam, Amsterdam Institute for Infection and Immunity, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam, NetherlandsDepartment of Molecular Cell Biology and Immunology, Cancer Center Amsterdam, Amsterdam Institute for Infection and Immunity, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam, NetherlandsDepartment of Surgery, Cancer Center Amsterdam, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam, NetherlandsInstitute of Immunology, Centre for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, AustriaDepartment of Medical Oncology, Cancer Center Amsterdam, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam, NetherlandsDepartment of Medical Oncology, Cancer Center Amsterdam, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam, NetherlandsDepartment of Molecular Cell Biology and Immunology, Cancer Center Amsterdam, Amsterdam Institute for Infection and Immunity, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam, NetherlandsDepartment of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, Netherlands0Department of Biomaterials, Science and Technology, Faculty of Science and Technology, University of Twente, Enschede, Netherlands1Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeDepartment of Molecular Cell Biology and Immunology, Cancer Center Amsterdam, Amsterdam Institute for Infection and Immunity, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam, NetherlandsDepartment of Molecular Cell Biology and Immunology, Cancer Center Amsterdam, Amsterdam Institute for Infection and Immunity, Amsterdam UMC, Vrije Universiteit Amsterdam, Amsterdam, NetherlandsMonocytes are antigen-presenting cells (APCs) that play diverse roles in promoting or regulating inflammatory responses, but their role in T cell stimulation is not well defined. In inflammatory conditions, monocytes frequently show increased expression of CD169/Siglec-1, a type-I interferon (IFN-I)-regulated protein. However, little is known about the phenotype and function of these CD169+ monocytes. Here, we have investigated the phenotype of human CD169+ monocytes in different diseases, their capacity to activate CD8+ T cells, and the potential for a targeted-vaccination approach. Using spectral flow cytometry, we detected CD169 expression by CD14+ CD16- classical and CD14+ CD16+ intermediate monocytes and unbiased analysis showed that they were distinct from dendritic cells, including the recently described CD14-expressing DC3. CD169+ monocytes expressed higher levels of co-stimulatory and HLA molecules, suggesting an increased activation state. IFNα treatment highly upregulated CD169 expression on CD14+ monocytes and boosted their capacity to cross-present antigen to CD8+ T cells. Furthermore, we observed CD169+ monocytes in virally-infected patients, including in the blood and bronchoalveolar lavage fluid of COVID-19 patients, as well as in the blood of patients with different types of cancers. Finally, we evaluated two CD169-targeting nanovaccine platforms, antibody-based and liposome-based, and we showed that CD169+ monocytes efficiently presented tumor-associated peptides gp100 and WT1 to antigen-specific CD8+ T cells. In conclusion, our data indicate that CD169+ monocytes are activated monocytes with enhanced CD8+ T cell stimulatory capacity and that they emerge as an interesting target in nanovaccine strategies, because of their presence in health and different diseases.https://www.frontiersin.org/articles/10.3389/fimmu.2021.697840/fullmonocyteCD169antigen-presentationCD8+ T cellnanovaccinecancer |