Aged Gut Microbiota Contributes to Systemical Inflammaging after Transfer to Germ-Free Mice
Advanced age is associated with chronic low-grade inflammation, which is usually referred to as inflammaging. Elderly are also known to have an altered gut microbiota composition. However, whether inflammaging is a cause or consequence of an altered gut microbiota composition is not clear. In this s...
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doaj-4b89781d37d84a47976bbb1f8cf57c7c2020-11-25T00:01:27ZengFrontiers Media S.A.Frontiers in Immunology1664-32242017-11-01810.3389/fimmu.2017.01385293898Aged Gut Microbiota Contributes to Systemical Inflammaging after Transfer to Germ-Free MiceFloris Fransen0Floris Fransen1Adriaan A. van Beek2Adriaan A. van Beek3Theo Borghuis4Theo Borghuis5Sahar El Aidy6Floor Hugenholtz7Floor Hugenholtz8Christa van der Gaast – de Jongh9Huub F. J. Savelkoul10Marien I. De Jonge11Mark V. Boekschoten12Mark V. Boekschoten13Hauke Smidt14Hauke Smidt15Marijke M. Faas16Marijke M. Faas17Paul de Vos18Paul de Vos19Top Institute Food and Nutrition, Wageningen, NetherlandsDepartment of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, Groningen, NetherlandsTop Institute Food and Nutrition, Wageningen, NetherlandsCell Biology and Immunology Group, Wageningen University, Wageningen, NetherlandsTop Institute Food and Nutrition, Wageningen, NetherlandsDepartment of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, Groningen, NetherlandsMicrobial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Groningen, NetherlandsTop Institute Food and Nutrition, Wageningen, NetherlandsLaboratory of Microbiology, Wageningen University, Wageningen, NetherlandsLaboratory of Pediatric Infectious Diseases, Radboud University Medical Center, Nijmegen, NetherlandsCell Biology and Immunology Group, Wageningen University, Wageningen, NetherlandsLaboratory of Pediatric Infectious Diseases, Radboud University Medical Center, Nijmegen, NetherlandsTop Institute Food and Nutrition, Wageningen, NetherlandsNutrition, Metabolism and Genomics Group, Division of Human Nutrition, Wageningen University, Wageningen, NetherlandsTop Institute Food and Nutrition, Wageningen, NetherlandsLaboratory of Microbiology, Wageningen University, Wageningen, NetherlandsDepartment of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, Groningen, NetherlandsDepartment of Obstetrics and Gynaecology, University Medical Center Groningen, University of Groningen, Groningen, NetherlandsTop Institute Food and Nutrition, Wageningen, NetherlandsDepartment of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, Groningen, NetherlandsAdvanced age is associated with chronic low-grade inflammation, which is usually referred to as inflammaging. Elderly are also known to have an altered gut microbiota composition. However, whether inflammaging is a cause or consequence of an altered gut microbiota composition is not clear. In this study, gut microbiota from young or old conventional mice was transferred to young germ-free (GF) mice. Four weeks after gut microbiota transfer immune cell populations in spleen, Peyer’s patches, and mesenteric lymph nodes from conventionalized GF mice were analyzed by flow cytometry. In addition, whole-genome gene expression in the ileum was analyzed by microarray. Gut microbiota composition of donor and recipient mice was analyzed with 16S rDNA sequencing. Here, we show by transferring aged microbiota to young GF mice that certain bacterial species within the aged microbiota promote inflammaging. This effect was associated with lower levels of Akkermansia and higher levels of TM7 bacteria and Proteobacteria in the aged microbiota after transfer. The aged microbiota promoted inflammation in the small intestine in the GF mice and enhanced leakage of inflammatory bacterial components into the circulation was observed. Moreover, the aged microbiota promoted increased T cell activation in the systemic compartment. In conclusion, these data indicate that the gut microbiota from old mice contributes to inflammaging after transfer to young GF mice.http://journal.frontiersin.org/article/10.3389/fimmu.2017.01385/fullgut microbiomeimmune systeminflammaginggerm-free miceaging |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Floris Fransen Floris Fransen Adriaan A. van Beek Adriaan A. van Beek Theo Borghuis Theo Borghuis Sahar El Aidy Floor Hugenholtz Floor Hugenholtz Christa van der Gaast – de Jongh Huub F. J. Savelkoul Marien I. De Jonge Mark V. Boekschoten Mark V. Boekschoten Hauke Smidt Hauke Smidt Marijke M. Faas Marijke M. Faas Paul de Vos Paul de Vos |
spellingShingle |
Floris Fransen Floris Fransen Adriaan A. van Beek Adriaan A. van Beek Theo Borghuis Theo Borghuis Sahar El Aidy Floor Hugenholtz Floor Hugenholtz Christa van der Gaast – de Jongh Huub F. J. Savelkoul Marien I. De Jonge Mark V. Boekschoten Mark V. Boekschoten Hauke Smidt Hauke Smidt Marijke M. Faas Marijke M. Faas Paul de Vos Paul de Vos Aged Gut Microbiota Contributes to Systemical Inflammaging after Transfer to Germ-Free Mice Frontiers in Immunology gut microbiome immune system inflammaging germ-free mice aging |
author_facet |
Floris Fransen Floris Fransen Adriaan A. van Beek Adriaan A. van Beek Theo Borghuis Theo Borghuis Sahar El Aidy Floor Hugenholtz Floor Hugenholtz Christa van der Gaast – de Jongh Huub F. J. Savelkoul Marien I. De Jonge Mark V. Boekschoten Mark V. Boekschoten Hauke Smidt Hauke Smidt Marijke M. Faas Marijke M. Faas Paul de Vos Paul de Vos |
author_sort |
Floris Fransen |
title |
Aged Gut Microbiota Contributes to Systemical Inflammaging after Transfer to Germ-Free Mice |
title_short |
Aged Gut Microbiota Contributes to Systemical Inflammaging after Transfer to Germ-Free Mice |
title_full |
Aged Gut Microbiota Contributes to Systemical Inflammaging after Transfer to Germ-Free Mice |
title_fullStr |
Aged Gut Microbiota Contributes to Systemical Inflammaging after Transfer to Germ-Free Mice |
title_full_unstemmed |
Aged Gut Microbiota Contributes to Systemical Inflammaging after Transfer to Germ-Free Mice |
title_sort |
aged gut microbiota contributes to systemical inflammaging after transfer to germ-free mice |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2017-11-01 |
description |
Advanced age is associated with chronic low-grade inflammation, which is usually referred to as inflammaging. Elderly are also known to have an altered gut microbiota composition. However, whether inflammaging is a cause or consequence of an altered gut microbiota composition is not clear. In this study, gut microbiota from young or old conventional mice was transferred to young germ-free (GF) mice. Four weeks after gut microbiota transfer immune cell populations in spleen, Peyer’s patches, and mesenteric lymph nodes from conventionalized GF mice were analyzed by flow cytometry. In addition, whole-genome gene expression in the ileum was analyzed by microarray. Gut microbiota composition of donor and recipient mice was analyzed with 16S rDNA sequencing. Here, we show by transferring aged microbiota to young GF mice that certain bacterial species within the aged microbiota promote inflammaging. This effect was associated with lower levels of Akkermansia and higher levels of TM7 bacteria and Proteobacteria in the aged microbiota after transfer. The aged microbiota promoted inflammation in the small intestine in the GF mice and enhanced leakage of inflammatory bacterial components into the circulation was observed. Moreover, the aged microbiota promoted increased T cell activation in the systemic compartment. In conclusion, these data indicate that the gut microbiota from old mice contributes to inflammaging after transfer to young GF mice. |
topic |
gut microbiome immune system inflammaging germ-free mice aging |
url |
http://journal.frontiersin.org/article/10.3389/fimmu.2017.01385/full |
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