Carbamazepine treatment recovered low N-acetylaspartate+N-acetylaspartylglutamate (tNAA) levels in the megencephaly mouse BALB/cByJ-Kv1.1mceph/mceph
Megencephaly mice (BALB/cByJ-Kv1.1mceph/mceph) display excessive brain growth and seizures related to a mutation within the potassium channel gene Kv1.1 producing a malfunctioning protein. 1H Magnetic resonance spectroscopy (MRS) provides means to study brain transmitters and metabolites in vivo. We...
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doaj-4b8ae2d274c741dc8b0ed5a90810f45e2021-03-20T04:54:11ZengElsevierNeurobiology of Disease1095-953X2007-04-01261221228Carbamazepine treatment recovered low N-acetylaspartate+N-acetylaspartylglutamate (tNAA) levels in the megencephaly mouse BALB/cByJ-Kv1.1mceph/mcephEric Westman0Christian Spenger1Lars-Olof Wahlund2Catharina Lavebratt3Department of Neurobiology, Health Care Sciences and Society, Karolinska Institutet, Stockholm, SwedenDepartment of Clinical Science, Intervention and Technology, Karolinska Institutet, Stockholm, Sweden; Corresponding author. Experimental MR Research Center, Karolinska University Hospital, MR Center, N8, SE-171 76 Stockholm, Sweden. Fax: +46 8 517 761 11.Department of Neurobiology, Health Care Sciences and Society, Karolinska Institutet, Stockholm, SwedenDepartment of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, SwedenMegencephaly mice (BALB/cByJ-Kv1.1mceph/mceph) display excessive brain growth and seizures related to a mutation within the potassium channel gene Kv1.1 producing a malfunctioning protein. 1H Magnetic resonance spectroscopy (MRS) provides means to study brain transmitters and metabolites in vivo. We applied MRS to pinpoint differences in hippocampus between mceph/mceph and wild type (wt) mice. Carbamazepine (CBZ) protects against brain overgrowth in mceph/mceph. Therefore, the effects of durable oral CBZ treatment on the MR spectra were investigated. LCModel was used for spectra quantification and multivariate data analysis applied to detect group differences. mceph/mceph mice had lower levels of N-acetylaspartate+N-acetylaspartylglutamate (tNAA) and choline-containing (tCho) compounds compared to wt mice. Glutamate, glutamine, taurine and myo-inositol levels were similar in wt and mceph/mceph. Furthermore, CBZ treatment recovered tCho and tNAA levels in mceph/mceph. Thus, distinct differences in MRS spectra between mceph/mceph and wt mice were depicted and treatment effects of CBZ were monitored using MRS.http://www.sciencedirect.com/science/article/pii/S0969996106003263MRSLCModelPLS-DAEpilepsymceph/mcephCarbamazepine |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Eric Westman Christian Spenger Lars-Olof Wahlund Catharina Lavebratt |
spellingShingle |
Eric Westman Christian Spenger Lars-Olof Wahlund Catharina Lavebratt Carbamazepine treatment recovered low N-acetylaspartate+N-acetylaspartylglutamate (tNAA) levels in the megencephaly mouse BALB/cByJ-Kv1.1mceph/mceph Neurobiology of Disease MRS LCModel PLS-DA Epilepsy mceph/mceph Carbamazepine |
author_facet |
Eric Westman Christian Spenger Lars-Olof Wahlund Catharina Lavebratt |
author_sort |
Eric Westman |
title |
Carbamazepine treatment recovered low N-acetylaspartate+N-acetylaspartylglutamate (tNAA) levels in the megencephaly mouse BALB/cByJ-Kv1.1mceph/mceph |
title_short |
Carbamazepine treatment recovered low N-acetylaspartate+N-acetylaspartylglutamate (tNAA) levels in the megencephaly mouse BALB/cByJ-Kv1.1mceph/mceph |
title_full |
Carbamazepine treatment recovered low N-acetylaspartate+N-acetylaspartylglutamate (tNAA) levels in the megencephaly mouse BALB/cByJ-Kv1.1mceph/mceph |
title_fullStr |
Carbamazepine treatment recovered low N-acetylaspartate+N-acetylaspartylglutamate (tNAA) levels in the megencephaly mouse BALB/cByJ-Kv1.1mceph/mceph |
title_full_unstemmed |
Carbamazepine treatment recovered low N-acetylaspartate+N-acetylaspartylglutamate (tNAA) levels in the megencephaly mouse BALB/cByJ-Kv1.1mceph/mceph |
title_sort |
carbamazepine treatment recovered low n-acetylaspartate+n-acetylaspartylglutamate (tnaa) levels in the megencephaly mouse balb/cbyj-kv1.1mceph/mceph |
publisher |
Elsevier |
series |
Neurobiology of Disease |
issn |
1095-953X |
publishDate |
2007-04-01 |
description |
Megencephaly mice (BALB/cByJ-Kv1.1mceph/mceph) display excessive brain growth and seizures related to a mutation within the potassium channel gene Kv1.1 producing a malfunctioning protein. 1H Magnetic resonance spectroscopy (MRS) provides means to study brain transmitters and metabolites in vivo. We applied MRS to pinpoint differences in hippocampus between mceph/mceph and wild type (wt) mice. Carbamazepine (CBZ) protects against brain overgrowth in mceph/mceph. Therefore, the effects of durable oral CBZ treatment on the MR spectra were investigated. LCModel was used for spectra quantification and multivariate data analysis applied to detect group differences. mceph/mceph mice had lower levels of N-acetylaspartate+N-acetylaspartylglutamate (tNAA) and choline-containing (tCho) compounds compared to wt mice. Glutamate, glutamine, taurine and myo-inositol levels were similar in wt and mceph/mceph. Furthermore, CBZ treatment recovered tCho and tNAA levels in mceph/mceph. Thus, distinct differences in MRS spectra between mceph/mceph and wt mice were depicted and treatment effects of CBZ were monitored using MRS. |
topic |
MRS LCModel PLS-DA Epilepsy mceph/mceph Carbamazepine |
url |
http://www.sciencedirect.com/science/article/pii/S0969996106003263 |
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