FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells

FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells

Single molecule tracking of fluorescent proteins in live cells is temporally limited by fluorophore photobleaching. Here the authors show using fluorophore pairs that FRET competes with photobleaching to improve photostability and allow longer-term tracking of both single proteins and complexes.

Bibliographic Details
Main Authors: Srinjan Basu, Lisa-Maria Needham, David Lando, Edward J. R. Taylor, Kai J. Wohlfahrt, Devina Shah, Wayne Boucher, Yi Lei Tan, Lawrence E. Bates, Olga Tkachenko, Julie Cramard, B. Christoffer Lagerholm, Christian Eggeling, Brian Hendrich, Dave Klenerman, Steven F. Lee, Ernest D. Laue
Format: Article
Language:English
Published: Nature Publishing Group 2018-06-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-018-04486-0

Internet

https://doi.org/10.1038/s41467-018-04486-0

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