FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells
Single molecule tracking of fluorescent proteins in live cells is temporally limited by fluorophore photobleaching. Here the authors show using fluorophore pairs that FRET competes with photobleaching to improve photostability and allow longer-term tracking of both single proteins and complexes.
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2018-06-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-018-04486-0 |
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doaj-4ba0bd5ae2024d44b50386dc802df3dc2021-05-11T09:43:05ZengNature Publishing GroupNature Communications2041-17232018-06-019111410.1038/s41467-018-04486-0FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cellsSrinjan Basu0Lisa-Maria Needham1David Lando2Edward J. R. Taylor3Kai J. Wohlfahrt4Devina Shah5Wayne Boucher6Yi Lei Tan7Lawrence E. Bates8Olga Tkachenko9Julie Cramard10B. Christoffer Lagerholm11Christian Eggeling12Brian Hendrich13Dave Klenerman14Steven F. Lee15Ernest D. Laue16Department of Biochemistry, University of CambridgeDepartment of Chemistry, University of CambridgeDepartment of Biochemistry, University of CambridgeDepartment of Biochemistry, University of CambridgeDepartment of Biochemistry, University of CambridgeDepartment of Biochemistry, University of CambridgeDepartment of Biochemistry, University of CambridgeDepartment of Biochemistry, University of CambridgeDepartment of Biochemistry, University of CambridgeDepartment of Biochemistry, University of CambridgeWellcome Trust – MRC Stem Cell Institute, University of CambridgeMedical Research Council Human Immunology Unit and Wolfson Imaging Centre, Weatherall Institute of Molecular Medicine, University of OxfordMedical Research Council Human Immunology Unit and Wolfson Imaging Centre, Weatherall Institute of Molecular Medicine, University of OxfordDepartment of Biochemistry, University of CambridgeDepartment of Chemistry, University of CambridgeDepartment of Chemistry, University of CambridgeDepartment of Biochemistry, University of CambridgeSingle molecule tracking of fluorescent proteins in live cells is temporally limited by fluorophore photobleaching. Here the authors show using fluorophore pairs that FRET competes with photobleaching to improve photostability and allow longer-term tracking of both single proteins and complexes.https://doi.org/10.1038/s41467-018-04486-0 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Srinjan Basu Lisa-Maria Needham David Lando Edward J. R. Taylor Kai J. Wohlfahrt Devina Shah Wayne Boucher Yi Lei Tan Lawrence E. Bates Olga Tkachenko Julie Cramard B. Christoffer Lagerholm Christian Eggeling Brian Hendrich Dave Klenerman Steven F. Lee Ernest D. Laue |
| spellingShingle |
Srinjan Basu Lisa-Maria Needham David Lando Edward J. R. Taylor Kai J. Wohlfahrt Devina Shah Wayne Boucher Yi Lei Tan Lawrence E. Bates Olga Tkachenko Julie Cramard B. Christoffer Lagerholm Christian Eggeling Brian Hendrich Dave Klenerman Steven F. Lee Ernest D. Laue FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells Nature Communications |
| author_facet |
Srinjan Basu Lisa-Maria Needham David Lando Edward J. R. Taylor Kai J. Wohlfahrt Devina Shah Wayne Boucher Yi Lei Tan Lawrence E. Bates Olga Tkachenko Julie Cramard B. Christoffer Lagerholm Christian Eggeling Brian Hendrich Dave Klenerman Steven F. Lee Ernest D. Laue |
| author_sort |
Srinjan Basu |
| title |
FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells |
| title_short |
FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells |
| title_full |
FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells |
| title_fullStr |
FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells |
| title_full_unstemmed |
FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells |
| title_sort |
fret-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells |
| publisher |
Nature Publishing Group |
| series |
Nature Communications |
| issn |
2041-1723 |
| publishDate |
2018-06-01 |
| description |
Single molecule tracking of fluorescent proteins in live cells is temporally limited by fluorophore photobleaching. Here the authors show using fluorophore pairs that FRET competes with photobleaching to improve photostability and allow longer-term tracking of both single proteins and complexes. |
| url |
https://doi.org/10.1038/s41467-018-04486-0 |
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