Toll-like receptor-4, but not toll-like receptor-2 mediates secretion of tumour necrosis factor α and interleukin-8 in lipopolysaccharide-stimulated mouse mammary epithelial cells

Toll-like receptor-4, but not toll-like receptor-2 mediates secretion of tumour necrosis factor α and interleukin-8 in lipopolysaccharide-stimulated mouse mammary epithelial cells

Mammary epithelial cells (MECs) from Kunming mice were isolated and stimulated in vitro with 10 μg/mL of Escherichia coli lipopolysaccharide (LPS). The release of tumour necrosis factor α (TNF-α) and interleukin-8 (IL-8) into culture supernatants was measured by ELISA. Furthermore, blocking experime...

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Bibliographic Details
Main Authors: He Chang-Liang, Yi Qiong, Li Yuan-Fang, Yang Hang, Wang Lu
Format: Article
Language:English
Published: Sciendo 2013-09-01
Series:Bulletin of the Veterinary Institute in Pulawy
Subjects:
mouse
mammary epithelial cells
cytokine
tlr2
tlr4
Online Access:https://doi.org/10.2478/bvip-2013-0068

Internet

https://doi.org/10.2478/bvip-2013-0068

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