An Improved Transformation System for Cowpea (Vigna unguiculata L. Walp) via Sonication and a Kanamycin-Geneticin Selection Regime

An Improved Transformation System for Cowpea (Vigna unguiculata L. Walp) via Sonication and a Kanamycin-Geneticin Selection Regime

An improved cowpea transformation method utilizing Agrobacterium-mediated gene delivery to explants derived from the cotyledonary nodes of imbibed cowpea seed is described. The explants were regenerated following a sonication procedure and a stringent selection comprising alternating regimes of kana...

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Bibliographic Details
Main Authors: Bosibori Bett, Stephanie Gollasch, Andy Moore, Robert Harding, Thomas J. V. Higgins
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-02-01
Series:Frontiers in Plant Science
Subjects:
cowpea transformation
Agrobacterium
sonication
kanamycin-based selection
geneticin-based selection
cowpeas
Online Access:https://www.frontiersin.org/article/10.3389/fpls.2019.00219/full

Internet

https://www.frontiersin.org/article/10.3389/fpls.2019.00219/full

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