An Improved Transformation System for Cowpea (Vigna unguiculata L. Walp) via Sonication and a Kanamycin-Geneticin Selection Regime
An improved cowpea transformation method utilizing Agrobacterium-mediated gene delivery to explants derived from the cotyledonary nodes of imbibed cowpea seed is described. The explants were regenerated following a sonication procedure and a stringent selection comprising alternating regimes of kana...
Main Authors: | Bosibori Bett, Stephanie Gollasch, Andy Moore, Robert Harding, Thomas J. V. Higgins |
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Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2019-02-01
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Series: | Frontiers in Plant Science |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2019.00219/full |
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