Anaerobic Acidogenic Fermentation of Cellobiose by Immobilized Cells: Prediction of Organic Acids Production by Response Surface Methodology

Response surface methodology was used to derive a prediction model for organic acids production by anaerobic acidogenic fermentation of cellobiose, using a mixed culture immobilized on γ-alumina. Three parameters (substrate concentration, temperature, and initial pH) were evaluated. In order to dete...

Full description

Bibliographic Details
Main Authors:	Panagiota Tsafrakidou, Konstantina Tsigkou, Argyro Bekatorou, Maria Kanellaki, Athanasios A. Koutinas
Format:	Article
Language:	English
Published:	MDPI AG 2021-08-01
Series:	Processes
Subjects:	anaerobic acidogenesis cellobiose organic acids immobilized cells γ-alumina predictive model
Online Access:	https://www.mdpi.com/2227-9717/9/8/1441

id	doaj-4bf76e203d4d4aa9ae54c32101c13e7d
record_format	Article
spelling	doaj-4bf76e203d4d4aa9ae54c32101c13e7d2021-08-26T14:16:29ZengMDPI AGProcesses2227-97172021-08-0191441144110.3390/pr9081441Anaerobic Acidogenic Fermentation of Cellobiose by Immobilized Cells: Prediction of Organic Acids Production by Response Surface MethodologyPanagiota Tsafrakidou0Konstantina Tsigkou1Argyro Bekatorou2Maria Kanellaki3Athanasios A. Koutinas4Department of Chemistry, University of Patras, 26500 Patras, GreeceDepartment of Chemical Engineering, University of Patras, 26504 Patras, GreeceDepartment of Chemistry, University of Patras, 26500 Patras, GreeceDepartment of Chemistry, University of Patras, 26500 Patras, GreeceDepartment of Chemistry, University of Patras, 26500 Patras, GreeceResponse surface methodology was used to derive a prediction model for organic acids production by anaerobic acidogenic fermentation of cellobiose, using a mixed culture immobilized on γ-alumina. Three parameters (substrate concentration, temperature, and initial pH) were evaluated. In order to determine the limits of the parameters, preliminary experiments at 37 °C were conducted using substrates of various cellobiose concentrations and pH values. Cellobiose was used as a model sugar for subsequent experiments with lignocellulosic biomass. The culture was well adapted to cellobiose by successive subculturing at 37 °C in synthetic media (with 100:5:1 COD:N:P ratio). The experimental data of successive batch fermentations were fitted into a polynomial model for the total organic acids concentration in order to derive a predictive model that could be utilized as a tool to predict fermentation results when lignocellulosic biomass is used as a substrate. The quadratic effect of temperature was the most significant, followed by the quadratic effect of initial pH and the linear effect of cellobiose concentration. The results corroborated the validity and effectiveness of the model.https://www.mdpi.com/2227-9717/9/8/1441anaerobic acidogenesiscellobioseorganic acidsimmobilized cellsγ-aluminapredictive model
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Panagiota Tsafrakidou Konstantina Tsigkou Argyro Bekatorou Maria Kanellaki Athanasios A. Koutinas
spellingShingle	Panagiota Tsafrakidou Konstantina Tsigkou Argyro Bekatorou Maria Kanellaki Athanasios A. Koutinas Anaerobic Acidogenic Fermentation of Cellobiose by Immobilized Cells: Prediction of Organic Acids Production by Response Surface Methodology Processes anaerobic acidogenesis cellobiose organic acids immobilized cells γ-alumina predictive model
author_facet	Panagiota Tsafrakidou Konstantina Tsigkou Argyro Bekatorou Maria Kanellaki Athanasios A. Koutinas
author_sort	Panagiota Tsafrakidou
title	Anaerobic Acidogenic Fermentation of Cellobiose by Immobilized Cells: Prediction of Organic Acids Production by Response Surface Methodology
title_short	Anaerobic Acidogenic Fermentation of Cellobiose by Immobilized Cells: Prediction of Organic Acids Production by Response Surface Methodology
title_full	Anaerobic Acidogenic Fermentation of Cellobiose by Immobilized Cells: Prediction of Organic Acids Production by Response Surface Methodology
title_fullStr	Anaerobic Acidogenic Fermentation of Cellobiose by Immobilized Cells: Prediction of Organic Acids Production by Response Surface Methodology
title_full_unstemmed	Anaerobic Acidogenic Fermentation of Cellobiose by Immobilized Cells: Prediction of Organic Acids Production by Response Surface Methodology
title_sort	anaerobic acidogenic fermentation of cellobiose by immobilized cells: prediction of organic acids production by response surface methodology
publisher	MDPI AG
series	Processes
issn	2227-9717
publishDate	2021-08-01
description	Response surface methodology was used to derive a prediction model for organic acids production by anaerobic acidogenic fermentation of cellobiose, using a mixed culture immobilized on γ-alumina. Three parameters (substrate concentration, temperature, and initial pH) were evaluated. In order to determine the limits of the parameters, preliminary experiments at 37 °C were conducted using substrates of various cellobiose concentrations and pH values. Cellobiose was used as a model sugar for subsequent experiments with lignocellulosic biomass. The culture was well adapted to cellobiose by successive subculturing at 37 °C in synthetic media (with 100:5:1 COD:N:P ratio). The experimental data of successive batch fermentations were fitted into a polynomial model for the total organic acids concentration in order to derive a predictive model that could be utilized as a tool to predict fermentation results when lignocellulosic biomass is used as a substrate. The quadratic effect of temperature was the most significant, followed by the quadratic effect of initial pH and the linear effect of cellobiose concentration. The results corroborated the validity and effectiveness of the model.
topic	anaerobic acidogenesis cellobiose organic acids immobilized cells γ-alumina predictive model
url	https://www.mdpi.com/2227-9717/9/8/1441
work_keys_str_mv	AT panagiotatsafrakidou anaerobicacidogenicfermentationofcellobiosebyimmobilizedcellspredictionoforganicacidsproductionbyresponsesurfacemethodology AT konstantinatsigkou anaerobicacidogenicfermentationofcellobiosebyimmobilizedcellspredictionoforganicacidsproductionbyresponsesurfacemethodology AT argyrobekatorou anaerobicacidogenicfermentationofcellobiosebyimmobilizedcellspredictionoforganicacidsproductionbyresponsesurfacemethodology AT mariakanellaki anaerobicacidogenicfermentationofcellobiosebyimmobilizedcellspredictionoforganicacidsproductionbyresponsesurfacemethodology AT athanasiosakoutinas anaerobicacidogenicfermentationofcellobiosebyimmobilizedcellspredictionoforganicacidsproductionbyresponsesurfacemethodology
_version_	1721190375287160832

Anaerobic Acidogenic Fermentation of Cellobiose by Immobilized Cells: Prediction of Organic Acids Production by Response Surface Methodology

Similar Items