Protocol for visualizing newly synthesized proteins in primary mouse hepatocytes

Protocol for visualizing newly synthesized proteins in primary mouse hepatocytes

Summary: Selective identification of newly synthesized proteins is challenging because all proteins, both existing and nascent, have the same amino acid pool and are therefore chemically indistinguishable. L-homopropargylglycine is an amino acid analog of methionine containing an alkyne moiety that...

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Bibliographic Details
Main Authors: Yuqian Shen, Wenhua Liu, Jian Zuo, Junhai Han, Zi Chao Zhang
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Cell culture
Cell isolation
Cell-based Assays
Microscopy
Gene Expression
Molecular/Chemical Probes
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721003233

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http://www.sciencedirect.com/science/article/pii/S2666166721003233

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