Observed crowding effects on Mycobacterium tuberculosis 2-trans-enoyl-ACP (CoA) reductase enzyme activity are not due to excluded volume only

Abstract The cellular milieu is a complex and crowded aqueous solution. Macromolecular crowding effects are commonly studied in vitro using crowding agents. The aim of the present study was to evaluate the effects, if any, of macromolecular synthetic crowding agents on the apparent steady-state kine...

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Bibliographic Details
Main Authors: Mariane Rotta, Luis F. S. M. Timmers, Carlos Sequeiros-Borja, Cristiano V. Bizarro, Osmar N. de Souza, Diogenes S. Santos, Luiz A. Basso
Format: Article
Language:English
Published: Nature Publishing Group 2017-07-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-017-07266-w