Microbial characterization and diversity of artisanal Ranchero cheese with emphasis in Lactococcus strains
Abstract The aim of this work is to characterize the natural microflora of artisanal Ranchero cheese and to identify Lactococcus isolates. Ten artisanal Ranchero cheese samples made with raw milk were obtained from local producers located in different geographical areas. Aerobic mesophilic, Staphyl...
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Sociedade Brasileira de Ciência e Tecnologia de Alimentos
2018-10-01
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doaj-4c4b84782adc42bb89824e12e39f72782020-11-24T20:47:12ZengSociedade Brasileira de Ciência e Tecnologia de AlimentosFood Science and Technology1678-457X2018-10-01010.1590/fst.28217S0101-20612018005024102Microbial characterization and diversity of artisanal Ranchero cheese with emphasis in Lactococcus strainsMario-Alejandro GALLEGOS-ACEVEDOAmérica CHÁVEZ-MARTÍNEZAgustín CORRAL-LUNAAna-Luisa RENTERÍA-MONTERRUBIOM. Eduvigues BURROLA-BARRAZARuth LECHUGA-VALLESJoel DOMINGUEZ-VIVEROSAlva-Rocío CASTILLO-GONZÁLEZRogelio SÁNCHEZ-VEGAAbstract The aim of this work is to characterize the natural microflora of artisanal Ranchero cheese and to identify Lactococcus isolates. Ten artisanal Ranchero cheese samples made with raw milk were obtained from local producers located in different geographical areas. Aerobic mesophilic, Staphylococci, Psychrophilic , total coliforms, molds, yeast and, lactic acid bacteria (LAB) (Lactococcus , Streptococcus mesophilic and thermophilic, Lactobacillus , Enterococcus, Leuconostoc) were numbered through agar plating. LAB isolates were classified by genus. Then, nineteen randomly selected presumptive Lactococcus isolates were assigned to specie by PCR amplification and DNA sequence. A high number of aerobic mesophilic Staphylococci, Psychrophilic, total coliforms, molds, and yeast were found. Lactococci, mesophilic and thermophilic Streptococci and Lactobacilli were the dominant LAB. Enterococci and Leuconostoc were also present. The isolates were identified as L. lactis subsp. lactis and L. garvieae . And both were found to be the dominant Lactococcus species that could be selected and used in a starter culture. Sanitation deficiencies in the production of artisanal Ranchero cheese were evident, which may translate into being a potential consumers health risk factor.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612018005024102&lng=en&tlng=enLactococcusmicrobiotaartisanal Ranchero cheese |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mario-Alejandro GALLEGOS-ACEVEDO América CHÁVEZ-MARTÍNEZ Agustín CORRAL-LUNA Ana-Luisa RENTERÍA-MONTERRUBIO M. Eduvigues BURROLA-BARRAZA Ruth LECHUGA-VALLES Joel DOMINGUEZ-VIVEROS Alva-Rocío CASTILLO-GONZÁLEZ Rogelio SÁNCHEZ-VEGA |
spellingShingle |
Mario-Alejandro GALLEGOS-ACEVEDO América CHÁVEZ-MARTÍNEZ Agustín CORRAL-LUNA Ana-Luisa RENTERÍA-MONTERRUBIO M. Eduvigues BURROLA-BARRAZA Ruth LECHUGA-VALLES Joel DOMINGUEZ-VIVEROS Alva-Rocío CASTILLO-GONZÁLEZ Rogelio SÁNCHEZ-VEGA Microbial characterization and diversity of artisanal Ranchero cheese with emphasis in Lactococcus strains Food Science and Technology Lactococcus microbiota artisanal Ranchero cheese |
author_facet |
Mario-Alejandro GALLEGOS-ACEVEDO América CHÁVEZ-MARTÍNEZ Agustín CORRAL-LUNA Ana-Luisa RENTERÍA-MONTERRUBIO M. Eduvigues BURROLA-BARRAZA Ruth LECHUGA-VALLES Joel DOMINGUEZ-VIVEROS Alva-Rocío CASTILLO-GONZÁLEZ Rogelio SÁNCHEZ-VEGA |
author_sort |
Mario-Alejandro GALLEGOS-ACEVEDO |
title |
Microbial characterization and diversity of artisanal Ranchero cheese with emphasis in Lactococcus strains |
title_short |
Microbial characterization and diversity of artisanal Ranchero cheese with emphasis in Lactococcus strains |
title_full |
Microbial characterization and diversity of artisanal Ranchero cheese with emphasis in Lactococcus strains |
title_fullStr |
Microbial characterization and diversity of artisanal Ranchero cheese with emphasis in Lactococcus strains |
title_full_unstemmed |
Microbial characterization and diversity of artisanal Ranchero cheese with emphasis in Lactococcus strains |
title_sort |
microbial characterization and diversity of artisanal ranchero cheese with emphasis in lactococcus strains |
publisher |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
series |
Food Science and Technology |
issn |
1678-457X |
publishDate |
2018-10-01 |
description |
Abstract The aim of this work is to characterize the natural microflora of artisanal Ranchero cheese and to identify Lactococcus isolates. Ten artisanal Ranchero cheese samples made with raw milk were obtained from local producers located in different geographical areas. Aerobic mesophilic, Staphylococci, Psychrophilic , total coliforms, molds, yeast and, lactic acid bacteria (LAB) (Lactococcus , Streptococcus mesophilic and thermophilic, Lactobacillus , Enterococcus, Leuconostoc) were numbered through agar plating. LAB isolates were classified by genus. Then, nineteen randomly selected presumptive Lactococcus isolates were assigned to specie by PCR amplification and DNA sequence. A high number of aerobic mesophilic Staphylococci, Psychrophilic, total coliforms, molds, and yeast were found. Lactococci, mesophilic and thermophilic Streptococci and Lactobacilli were the dominant LAB. Enterococci and Leuconostoc were also present. The isolates were identified as L. lactis subsp. lactis and L. garvieae . And both were found to be the dominant Lactococcus species that could be selected and used in a starter culture. Sanitation deficiencies in the production of artisanal Ranchero cheese were evident, which may translate into being a potential consumers health risk factor. |
topic |
Lactococcus microbiota artisanal Ranchero cheese |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612018005024102&lng=en&tlng=en |
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