Development of a novel and economical agar-based non-adherent three-dimensional culture method for enrichment of cancer stem-like cells

Development of a novel and economical agar-based non-adherent three-dimensional culture method for enrichment of cancer stem-like cells

Abstract Background Non-adherent or ultra-low attachment three-dimensional (3D) culture, also called sphere formation assay, has been widely used to assess the malignant phenotype and stemness potential of transformed or cancer cells. This method is also popularly used to isolate the cancer stem-lik...

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Bibliographic Details
Main Authors: Weijie Gao, Dinglan Wu, Yuliang Wang, Zhu Wang, Chang Zou, Yong Dai, Chi-Fai Ng, Jeremy Yuen-Chun Teoh, Franky Leung Chan
Format: Article
Language:English
Published: BMC 2018-09-01
Series:Stem Cell Research & Therapy
Subjects:
3D culture
Agar gel
Anchorage-independent growth
Cancer stem-like cells
Prostatospheres
Tumor spheres
Online Access:http://link.springer.com/article/10.1186/s13287-018-0987-x

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http://link.springer.com/article/10.1186/s13287-018-0987-x

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