Development of a novel and economical agar-based non-adherent three-dimensional culture method for enrichment of cancer stem-like cells
Abstract Background Non-adherent or ultra-low attachment three-dimensional (3D) culture, also called sphere formation assay, has been widely used to assess the malignant phenotype and stemness potential of transformed or cancer cells. This method is also popularly used to isolate the cancer stem-lik...
Main Authors: | Weijie Gao, Dinglan Wu, Yuliang Wang, Zhu Wang, Chang Zou, Yong Dai, Chi-Fai Ng, Jeremy Yuen-Chun Teoh, Franky Leung Chan |
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Format: | Article |
Language: | English |
Published: |
BMC
2018-09-01
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Series: | Stem Cell Research & Therapy |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s13287-018-0987-x |
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