The Use of Ribosomal RNA as a Microbial Source Tracking Target Highlights the Assay Host-Specificity Requirement in Water Quality Assessments

The Use of Ribosomal RNA as a Microbial Source Tracking Target Highlights the Assay Host-Specificity Requirement in Water Quality Assessments

For microbial source tracking (MST), the 16S ribosomal RNA genes (rDNA) of host-specific bacteria and mitochondrial DNA (mtDNA) of animal species, known to cause fecal contamination of water, have been commonly used as molecular targets. However, low levels of contamination might remain undetected b...

Full description

Bibliographic Details
Main Authors: Annastiina Rytkönen, Ananda Tiwari, Anna-Maria Hokajärvi, Sari Uusheimo, Asko Vepsäläinen, Tiina Tulonen, Tarja Pitkänen
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-06-01
Series:Frontiers in Microbiology
Subjects:
microbial source tracking
performance analysis
field validation
ribosomal RNA
RT-qPCR
fecal contamination
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2021.673306/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2021.673306/full

Similar Items