Identification of novel esterase-active enzymes from hot environments by use of the host bacterium Thermus thermophilus

Identification of novel esterase-active enzymes from hot environments by use of the host bacterium Thermus thermophilus

Functional metagenomic screening strategies, which are independent of known sequence information, can lead to the identification of truly novel genes and enzymes. Since E. coli has been used exhaustively for this purpose as a host, it is important to establish alternative expression hosts and to use...

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Bibliographic Details
Main Authors: Benedikt eLeis, Angel eAngelov, Markus eMientus, Haijuan eLi, Vu Thuy Trang ePham, Benjamin eLauinger, Patrick eBongen, Jörg ePietruszka, Luís G. Gonçalves, Helena eSantos, Wolfgang eLiebl
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-04-01
Series:Frontiers in Microbiology
Subjects:
Thermus thermophilus
functional metagenomics
comparative screening
novel screening host
novel metagenomic esterases
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00275/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00275/full

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