Mutations in the C-terminus of HBoV NS1 affect the function of NP1
Abstract Human bocavirus 1 (HBoV1) is an autonomous parvovirus in the Bocaparvovirus genus. The multifunctional nuclear protein NP1 is involved in viral replication. In the present study, we found that the mutations in the C-terminus of NS1 affected NP1 function in viral replication. Knocking out NP...
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doaj-4c9ae7e602524ea99bc6368ba0148dc22020-12-08T01:53:18ZengNature Publishing GroupScientific Reports2045-23222017-08-017111210.1038/s41598-017-06513-4Mutations in the C-terminus of HBoV NS1 affect the function of NP1Junmei Zhang0Yan Bai1Bing Zhu2Sujuan Hao3Zhen Chen4Hanzhong Wang5Wuxiang Guan6Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of SciencesPediatric department of Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyCenter Laboratory, Guangzhou Women and Children’s Medical CenterCenter for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of SciencesCenter for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of SciencesCenter for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of SciencesCenter for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of SciencesAbstract Human bocavirus 1 (HBoV1) is an autonomous parvovirus in the Bocaparvovirus genus. The multifunctional nuclear protein NP1 is involved in viral replication. In the present study, we found that the mutations in the C-terminus of NS1 affected NP1 function in viral replication. Knocking out NP1 expression in the recombinant infectious clone, on which the C-terminus of NS1 was mutated based on the clinical samples from nasopharyngeal aspirates, resulted in different degrees of decreased replication. The result suggested that NP1 facilitated the replication of viral genome but was not necessary, which is different from the minute virus of canines, where NP1 is essential for viral replication. Further studies showed that clinical mutations in the NP1 region did not affect viral genome replication, and UP1 promoted viral DNA replication. Our results suggested that the C-terminus of NS1 is important for viral replication and may be a target for regulating the replication of the viral genome.https://doi.org/10.1038/s41598-017-06513-4 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Junmei Zhang Yan Bai Bing Zhu Sujuan Hao Zhen Chen Hanzhong Wang Wuxiang Guan |
spellingShingle |
Junmei Zhang Yan Bai Bing Zhu Sujuan Hao Zhen Chen Hanzhong Wang Wuxiang Guan Mutations in the C-terminus of HBoV NS1 affect the function of NP1 Scientific Reports |
author_facet |
Junmei Zhang Yan Bai Bing Zhu Sujuan Hao Zhen Chen Hanzhong Wang Wuxiang Guan |
author_sort |
Junmei Zhang |
title |
Mutations in the C-terminus of HBoV NS1 affect the function of NP1 |
title_short |
Mutations in the C-terminus of HBoV NS1 affect the function of NP1 |
title_full |
Mutations in the C-terminus of HBoV NS1 affect the function of NP1 |
title_fullStr |
Mutations in the C-terminus of HBoV NS1 affect the function of NP1 |
title_full_unstemmed |
Mutations in the C-terminus of HBoV NS1 affect the function of NP1 |
title_sort |
mutations in the c-terminus of hbov ns1 affect the function of np1 |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-08-01 |
description |
Abstract Human bocavirus 1 (HBoV1) is an autonomous parvovirus in the Bocaparvovirus genus. The multifunctional nuclear protein NP1 is involved in viral replication. In the present study, we found that the mutations in the C-terminus of NS1 affected NP1 function in viral replication. Knocking out NP1 expression in the recombinant infectious clone, on which the C-terminus of NS1 was mutated based on the clinical samples from nasopharyngeal aspirates, resulted in different degrees of decreased replication. The result suggested that NP1 facilitated the replication of viral genome but was not necessary, which is different from the minute virus of canines, where NP1 is essential for viral replication. Further studies showed that clinical mutations in the NP1 region did not affect viral genome replication, and UP1 promoted viral DNA replication. Our results suggested that the C-terminus of NS1 is important for viral replication and may be a target for regulating the replication of the viral genome. |
url |
https://doi.org/10.1038/s41598-017-06513-4 |
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