Molecular Attributes Associated With Refolding of Inclusion Body Proteins Using the Freeze–Thaw Method

Molecular Attributes Associated With Refolding of Inclusion Body Proteins Using the Freeze–Thaw Method

Understanding the structure–function of inclusion bodies (IBs) in the last two decades has led to the development of several mild solubilization buffers for the improved recovery of bioactive proteins. The recently developed freeze–thaw-based inclusion body protein solubilization method has received...

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Bibliographic Details
Main Authors: Priyank Singhvi, Juhi Verma, Neha Panwar, Tabiya Qayoom Wani, Akansha Singh, Md. Qudratullah, Arnab Chakraborty, Ankit Saneja, Debi P. Sarkar, Amulya K. Panda
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-04-01
Series:Frontiers in Microbiology
Subjects:
inclusion bodies
mild solubilization method
freeze–thaw method
protein refolding
human growth hormone
protein aggregates
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2021.618559/full

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https://www.frontiersin.org/articles/10.3389/fmicb.2021.618559/full

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