A fast, efficient and high-throughput procedure involving laser microdissection and RT droplet digital PCR for tissue-specific expression profiling of rice roots

A fast, efficient and high-throughput procedure involving laser microdissection and RT droplet digital PCR for tissue-specific expression profiling of rice roots

Abstract Background In rice, the cortex and outer tissues play a key role in submergence tolerance. The cortex differentiates into aerenchyma, which are air-containing cavities that allow the flow of oxygen from shoots to roots, whereas exodermis suberification and sclerenchyma lignification limit o...

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Bibliographic Details
Main Authors: Thibault Mounier, Sergi Navarro-Sanz, Charlotte Bureau, Lefeuvre Antoine, Fabrice Varoquaux, Franz Durandet, Christophe Périn
Format: Article
Language:English
Published: BMC 2020-12-01
Series:BMC Molecular and Cell Biology
Subjects:
Rice
Root meristem
Laser microdissection (LM)
Cortex
ddRT-PCR
Droplet digital PCR
Online Access:https://doi.org/10.1186/s12860-020-00312-y

Internet

https://doi.org/10.1186/s12860-020-00312-y

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